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How to Calculate the Amount of P-nitrophenol Produced
In my test tube I have 19mL of glycine buffer and 01mL of p-nitrophenol and then I am suppose to calculate how much p-nitrophenol in nmol is present. Working stock solution of 1mM pNP is. P Nitrophenol Standard Curve 2 9 Characterization Of Alkaline Download Scientific Diagram Determination of p-nitrophenol produced using a high and a low substrate concentration based on p-nitrophenol standards. . You start with 01 ml of 025mM 250 uM or 250000 nM p-NP final volume is 2 ml so you diluted it 20-fold. Mix the contents of each tube well. In this case the amount of p-Nitrophenol in a sample with an absorbance value of 073 is approximately 44 nmol. Calculate the nmol of p-nitrophenol produced in your reactions using Beers law A εcl and mathematical manipulation in the following way - divide the value obtained for absorbance by the extinction co-efficient of p-nitrophenol at 405 nm 102 mM-1 cm-1 and the path length of the cuvette...
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